Membrane Anchored Polymers Modulate Amyloid Fibrillation

Sen, N. et. al., Macromolecular Rapid Communication, 2021, 2100120,

We here report on the influence of conjugates fabricated from lipid anchors (cholesterol, diacylglycerol) and hydrophilic polymers on Aβ1-40 fibrillation, aiming to understand the impact of polymers cloud point temperature (Tcp) and its hydrophobic tails on the amyloid fibrillation. Novel lipid-polymer conjugates, consisting of poly(oligo(ethylene glycol)macrylates) and hydrophobic groups (diacylglyceryl-, cholesteryl-, octyl-, decyl-, hexadecyl-) as anchors are synthesized  via RAFT polymerization, allowing to tune the hydrophilic-hydrophobic profile of the conjugates. Hydrophobic lipid-anchors are significantly delaying fibrillation (both lag, tlag and half times, t1/2), observing similar fibrillar structures via TEM when compared to native Aβ1-40. © 2021 The Authors. Macromolecular Rapid Communications published by Wiley‐VCH GmbH