Hoffmann, J.F., et al. Angewandte Chemie, International Edition, 2021, accepted, https://doi.org/10.1002/anie.202015179
The internal structure of single-chain nanoparticles (SCNPs) has been investigated, forming nanosized domains preformed during the crosslinking process. We present proof for the presence of nano-compartments within SCNPs via a combination of electron paramagnetic resonance (EPR) and fluorescence spectroscopy. A novel strategy to encapsulate labels within such water dispersible SCNPs is presented.