Inhibition of the fibrillation of amyloid Aβ1-40 by hybrid- lipid-polymer vesicles

Sen, N. et al., Macromolecular Bioscience, 2023, 2200522, DOI:10.1002/mabi.202200522

The transformation of functional proteins into amyloidic plaques is responsible for the impairment of neurological functions in patients fallen victim to debilitating neurological conditions like Alzheimer’s, Parkinson’s, and Huntington’s diseases. We investigate the influence of lipid hybrid-vesicles on alterations of the nucleation process and thus early phases of Aβ1-40 fibrillation. Hybrid-vesicles (±100 nm) are prepared by incorporating variable amounts of cholesterol-/glycerol-conjugated poly(di(ethylene glycol)macrylates)n polymers into 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) membranes. Such polymers, when embedded in hybrid-vesicles (up to 20%) significantly prolonged the fibrillation lag phase (tlag) compared to a small acceleration in the presence of DOPC vesicles, irrespective of the amount of polymers inside the hybrid-vesicles. © 2023 The Authors. Macromolecular Bioscience published by Wiley‐VCH GmbH.